Figure 1.

CRISPR-Cas9 targeting of different HIV-1 isolates. (a) The HIV-1 proviral genome with the position of the gRNA-target sites indicated. The gRNAs binding to the sense and antisense DNA strand are indicated above and below the proviral genome, respectively. (b) Different HIV-1 isolates tested in this study. For each isolate, the subtype, source (PI, primary isolate; LA, laboratory-adapted) and tropism (X4, CXCR4-using; R5, CCR5-using) are indicated. The gRNA-target sequence is shown with nucleotides mismatching with the gRNA in red and underlined (number of mismatching nt indicated between brackets) and the PAM nucleotides in bold. The Cas9 cleavage site is indicated with a red arrow. (c) Experimental design. See text for details.