Figure 2.

Replication of HIV-1 isolates in Cas9-gRNA-expressing T cells. Jurkat T-cells stably transduced with lentiviral vectors expressing Cas9 and single or dual gRNAs were infected with the different HIV-1 isolates and cultured for up to 110 days. (a–g) The CA-p24 level in the culture supernatant was measured to monitor virus replication. All viruses were tested in four cultures and one representative culture is shown. (h) Replication in the presence of the antiviral gRNAs was compared to replication in control gLuc-expressing cells (as shown in a–g). The color indicates the effect of the gRNAs on virus replication (orange, no inhibition; yellow, delayed replication; green, no replication). The number of nt mismatches in the viral targets are shown (*, nt mismatch at Cas9 cleavage site).