Table 1.
Virus rescue assay.
| Virus | Cultures with Replication-Competent Virus 1 | ||||
|---|---|---|---|---|---|
| gGag1 + gEnv2 | gGag1 + gTatRev | ||||
| Subtype | Isolate | day 20 | day 110 | day 20 | day 110 |
| B | LAI | 1/4 | 0/4 | 4/4 | NT |
| B | JR-CSF | 0/4 | 0/4 | 1/4 | 0/4 |
| B | NL4-3 | 1/4 | 0/4 | 4/4 | NT |
| A | 92UG029 | 1/4 | 0/4 | 4/4 | NT |
| C | PHD79B8 | NT | NT | NT | NT |
| D | 92UG024 | NT | NT | 0/4 | 0/4 |
| AE | 94TH304 | 0/4 | 0/4 | 4/4 | NT |
1 Infected dual-gRNA cell cultures that did not (or not yet) demonstrate virus replication were mixed with an equal amount of wild-type, unprotected cells at 20 and 110 days after infection. Replication in these mixed cell cultures was monitored by CA-p24 ELISA to detect rescue of replication-competent virus. For each virus, all four infected cell cultures were tested and the fraction of cultures that scored positive, i.e. in which CA-p24 was detectable within the 21-day follow-up period, is indicated. NT: not tested because of breakthrough virus replication at the indicated time.