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. 2019 Mar 19;11(3):278. doi: 10.3390/v11030278

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Subcellular localization of differential expression gene involved in SsHADV-1 virions infection. (A) Heat map enrichment analysis of all differentially expressed genes. Row clustering was carried out based on subcellular localization. The column represents the individual treatment sample. Down-regulated DEGs are displayed in green color, and up-regulated DEGs in red. The brightness of each color corresponds to the magnitude of the difference when compared against the average value. (B) Histogram of differentially expressed genes in various organelles, which respectively shows the number of up-regulated (red) and down-regulated (green) genes in each cell structure. (C) RT-qPCR validated the expression of cell membrane-related DEGs (seven up-regulated and three down-regulated DEGs). Three technical replicates were performed for each RT-qPCR. The ubiquitin gene was used as a reference gene.