Figure 4.

Non-degradative steps of autophagy increase both LASV and MOPV infectious particle production. (A) HeLa cells were transfected with the indicated siRNA for 72 h before analysis of silencing efficiency by Western blotting (n = 4 independent experiments); (B–D) the same cells as in (A) were then infected with LASV or MOPV at an MOI of 0.1 for 1 h before being maintained for three days at 37 °C. Viral RNA was then extracted from the cells and supernatants for quantification by RTqPCR. The infectious particles from supernatants were also titrated on Vero cells. The error bars represent the standard error of the means from four independent experiments. * indicates p < 0.05, as determined by the Mann–Whitney test.