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. 2019 Mar 19;16(6):983. doi: 10.3390/ijerph16060983

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemistry for oligodendrocytes (brown) in the cerebellum (A–C) of offspring from CTL, Pb, and PA groups. Note that the numbers of Olig2-positive oligodendrocytes in the cerebellum are reduced in the Pb group, and ascorbic acid treatment ameliorated these reductions in the PA group. GM-GCL (blue), granule cell layer in gray matter; WM, white matter. Bar = 25 μm. (D) The numbers of OPN-positive Purkinje cells in the cerebellar cortex are expressed as percentages of the value in the CTL group (n = 12 offspring per group; ^a p < 0.05, indicating asignificant difference compared with the control group, ^b p < 0.05, indicating a significant difference compared with the Pb group). The bars indicate the means ± standard errors of the mean.