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. 2019 Mar 14;62(7):3539–3552. doi: 10.1021/acs.jmedchem.8b02026

Table 3. Functional Analysis of hA3AR Antagonism from [35S]GTPγS Binding Assaysa.

  preincubation
co-incubation
 
compound pA2 Schild slope pA2 Schild slope mode of antagonism
17b NA NA 7.4 ± 0.1 1.1 ± 0.1 competitive insurmountable
19 5.9 ± 0.1 1.1 ± 0.1 6.2 ± 0.1 1.0 ± 0.1 competitive surmountable
a

Values represent mean ± SEM of three separate experiments each performed in duplicate.