Table 3. Functional Analysis of hA3AR Antagonism from [35S]GTPγS Binding Assaysa.
preincubation |
co-incubation |
||||
---|---|---|---|---|---|
compound | pA2 | Schild slope | pA2 | Schild slope | mode of antagonism |
17b | NA | NA | 7.4 ± 0.1 | 1.1 ± 0.1 | competitive insurmountable |
19 | 5.9 ± 0.1 | 1.1 ± 0.1 | 6.2 ± 0.1 | 1.0 ± 0.1 | competitive surmountable |
Values represent mean ± SEM of three separate experiments each performed in duplicate.