LSTDO attenuates the
inflammation induced by DNA origami. (A) ELISA
test on FVB/N mice. Mice (two groups of four mice) were treated with
STDO, LSTDO, and liposomes to detect acute inflammation. After 6 h,
blood was collected and analyzed: IL-6 increased in mice treated with
STDO (*p-value < 0.05). (B) Evaluation of interferon
activation in THP1 monocytic cell line. THP1 cell line was modified
in order to secrete a luciferase under the control of interferon pathway.
Cells were treated with 10 and 100 μg/mL of STDO, LSTDO, and
liposomes. Interferon production was enhanced by the treatment with
STDO (**p-value < 0.01; * <0.05). (C) Schematic
representation of the TLR9 stimulation by STDO and LSTDO.