Figure 2.

Representation of clustered regularly interspaced short palindromic repeats (CRISPR) Cas9-mediated gene editing. Upon recognising an adequate PAM site (red), the targeting sequence (blue) of gRNA (yellow) begins to anneal to the target DNA in the 3′-5′ direction. If enough homology between strands exists, Cas9 undergoes a conformational change that ultimately results in a blunt DSB.