Table 3.
Heatmap of estimated endolysin C-termini charges over the pH range and the residual antibacterial activity within this range (http://protcalc.sourceforge.net/). Min to Max pKa and residual activity is indicated with the color scale from red to green background. For all experiments, the mean values of three independent experiments are shown.
| pH | C-Termini Charge (pKa) | Residual Antibacterial Activity, % | |||||
|---|---|---|---|---|---|---|---|
| LysAm24 | LysECD7 | LysSi3 | LysAm24 | LysECD7 | LysSi3 | ||
| 5.00 | 12.80 | 8.00 | 13.80 | 98.8 | 100.0 | 99.8 | |
| 5.50 | 11.60 | 7.30 | 12.60 | 99.,4 | 100.0 | 100.0 | |
| 6.00 | 10.20 | 6.10 | 11.20 | 97.2 | 97.6 | 99.9 | |
| 6.50 | 8.00 | 4.00 | 9.00 | 55.0 | 75.0 | 57.2 | |
| 7.00 | 5.80 | 1.80 | 6.80 | 45.0 | 62.5 | 29.2 | |
| 7.50 | 4.40 | 0.50 | 5.40 | 62.5 | 94.5 | 88.5 | |
| 8.00 | 3.40 | −0.30 | 4.40 | 57.5 | 96.5 | 98.8 | |
| 8.50 | 2.60 | −0.70 | 3.50 | 16.0 | 90.0 | 69.0 | |
| 9.00 | 1.80 | −1.00 | 2.50 | 45.0 | 50.0 | 65.0 | |