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. 2019 Mar 26;16(6):1085. doi: 10.3390/ijerph16061085

Figure 1.

Figure 1

Cell viability and cytokine/chemokine release after exposure to spores and hyphal fragments from various mold species. BEAS-2B cells were exposed to 100 µg dry weight/mL of X-ray treated aerosolized spores (as), washed spores (ws) or hyphal fragments (hf) from A. fumigatus (AF), P. chrysogenum (PC), A. versicolor (AV), S. chararum (SC) or medium (control) for 6 h, and assessed for: (A) cytotoxicity by staining with PI and Hoechst and analyzed with fluorescence microscopy. The results represent mean ± SEM of >3 independent experiments. Statistical analyses were performed by one-way ANOVA with Dunnett’s post-tests on log transformed data. Significant difference compared to the control denoted by * p < 0.05, or (B) IL-6 and IL-8 release measured by ELISA. Bars represent mean ± SEM concentrations of >6 independent experiments. Statistical analyses were performed by two-way ANOVA with Dunnett’s post-tests on 2-step normalized data. Significant difference compared to the control denoted by * p < 0.05.