Figure 5.

Inhibition of A. fumigatus- or P. chrysogenum-induced IL-6 and IL-8 release by attenuating TLR2 and TLR4. BEAS-2B cells were pretreated with human TLR2 blocking antibody MAB2616 (0.2 µg/mL) or TLR4 antagonist AF1478 (10 µg/mL) for one h and further incubated with X-ray treated hyphal fragments (hf) of A. fumigatus (AF; 10 µg dry weight/mL) or P. chrysogenum (PC; 100 µg dry weight/mL) for 6 h. Medium blanks were included as control. Release of (A) IL-6 and (B) IL-8 were assessed by ELISA. Bars represent mean ± SEM of three independent experiments. Statistical analyses were performed by two-way ANOVA with Dunnett’s post-tests on 2-step normalized data. Significant difference compared to the respective hyphal fragments denoted by * p < 0.05.