Figure 7.

Time-dependent expression and release of IL-6 and IL-8 after exposure to untreated spores and hyphal fragments from A. fumigatus. Cells were exposed to untreated aerosolized spores (as) or hyphal fragments (hf) of A. fumigatus (67 µg dry weight/mL) for three different time points. Medium blanks were included as control. (A) Expression of IL-6 and IL-8 were measured by real-time RT-PCR. (B) Release of IL-6 and IL-8 were measured with ELISA. Bars represent mean ± SEM of <4 independent experiments. Statistical analyses were performed by two-way ANOVA with Dunnett’s post-tests on log transformed or 2-step normalized data respectively. Significant difference compared to the control denoted by * p < 0.05.