Fig. 6.

The effect of CHC on the cellular uptake of AR-C155858 and AZD3965 in 4T1 cells. The uptake of AR-C155858 (a) and AZD3965 (b) in the absence and presence of CHC (20 mM) at physiological pH (serum-free medium). Cells were pre-incubated with CHC for 30 min at 37 °C followed by uptake of AR-C155858 or AZD3965 at RT for 2 or 5 min, respectively. NS nonsignificant; **P < 0.01; ***P < 0.001; ****P < 0.0001 compared to the uptake AR-C155858 or AZD3965 in the absence of CHC. Two-way ANOVA followed by Bonferroni’s post hoc test. Data are presented as mean ± SD, n = 3