Table 1 |.

IcsB substrates identified in chemical proteomic screens

Protein symbol	IcsB transfection	S. flexneri WT infection	S. flexneri ΔvirA infection	Loss of function test	Lipidation	Classification
RHOA, RHOC, RHOG, RAC1	√	√	√	Knockout	*	Rho family
RHOF		√	√	Knockout	*
RAC2	√			Not tested	*
CDC42		√	√	Knockout	*
H-RAS, K-RAS, R-RAS, RAP1B	√	√	√	Knockout	*	Ras family
RALA	√	√	√	Not tested	*
RALB	√	√		Not tested	*
RAP1A		√	√	Knockout	*
RHEB	√			Not tested	*
RAB8A, RAB11A, RAB11B, RAB13,	√	√	√	Knockout	*	Rab family
RAB22A, RAB23, RAB34, RAB35
RAB5B	√		√	Not tested	*
RAB6A		√		Knockout	*
RAB6B		√		Knockout	*
RAB9A		√	√	Knockout	*
RAB39A		√		Not tested	*
YKT6	√	√	√	RNAi (85%)	*	SNARE
VAMP3	√			RNAi (89%)
VAMP8	√	√	√	Knockout
BNIP1	√			Not tested
SEC22B	√			RNAi (95%)
SEPT2		√		Not tested		Septin
SEPT7	√	√	√	RNAi (70%)
SEPT9		√		Not tested
SEPT11		√	√	Knockout
CHMP5	√	√	√	Knockout
CHMP4B	√			Not tested
BASP1	√	√	√	Not tested	*
MARCKS	√	√	√	Knockout	*
MARCKSL1	√	√	√	Not tested	*
CNP	√	√	√	Knockout	*
GNG12	√	√		Not tested	*
CD44		√	√	Knockout
CDCP1		√	√	Not tested
DSG2		√	√	Knockout
FNBP1L		√	√	Knockout
WASL		√	√	Not tested
TRIP10		√	√	Knockout
FERMT2			√	Not tested
EPS8			√	Not tested
EPS8L2		√		Not tested
S100A13	√			Not tested
PTRF	√			Not tested
GIPC1	√			Not tested
FKBP8	√			RNAi (95%)
FKBP11	√			Not tested
EPB41L2	√			Not tested
EPB41L1	√			Not tested

RNAi, RNA interference.