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. Author manuscript; available in PMC: 2019 Oct 19.
Published in final edited form as: ACS Chem Biol. 2018 Oct 4;13(10):3011–3020. doi: 10.1021/acschembio.8b00804

Figure 2. Pyp1 hydrolyzes (D)-polyol phosphates.

Figure 2.

(A). Purified Pypl’s phosphatase activity was assayed against 0.5 mM ribitol-5P by LC-MS. Incubation with Pyp1 led to the depletion of ribitol-5P (left panel) and the accumulation of ribitol (right panel). (B). Pyp1’s (D)-polyol phosphatase activity was measured in the presence of 0.5 mM substrate and 5 mM Mg2+ (pH = 7.0, 30°C) by monitoring the appearance the polyol. See Experimental Procedures for details. The y-axis represents specific phosphatase activity (μmoles product per min per mg of protein) (mean ± range, N = 2).