Fig. 4.

Depletion of PCAT-1 activates p38 MAPK through ASK1 and induces Caspase mediated apoptosis in HNSCC. Protein lysates from JHU029 and Cal27 cells transfected with control or siRNA to PCAT-1 were subjected to Western blot analysis using specific antibodies. a: Expression of phospho-ASK1 and total ASK1 and the right panel shows quantitation of the data using Image J Software b: Expression of phospho-p38 and total p38 and right panel shows quantitation of the data. The membranes were reprobed with antibody to GAPDH as an internal control. Small bar indicates standard error (*, p < 0.05; ** p < 0.01). c: Western blot analysis of the cell lysates was performed using the specific antibodies to PARP or Caspase 9. The membrane was reprobed with antibody to GAPDH as an internal control