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. 2019 Apr 15;19:21. doi: 10.1186/s12896-019-0515-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Simplified DNA plasmid map of lentivirus transfer vector TRE3G-cMyc. The vector is a third-generation lentivirus transfer vector containing the third-generation tetracycline responsive element (TRE3G) controlling the expression of FLAG tagged c-Myc (mouse) transcription factor, constitutive expression of the rtTA, and a puromycin (puro) resistance gene. Depicted is the rtTA transcription factor activated by soluble dox binding to the TRE3G promoter