Fig. 4.

Induced proliferation of lin⁻Ter119⁻Mac-1⁻Gr-1⁻c-Kit⁺CD71^(low/−) mouse bone marrow cells by over-expression of the transcription factor c-Myc. Shown are average measurements from 3 separate in vitro experiments where cells were modified by stable gene transfer of the TRE3G-cMyc vector, cultured in puromycin to eliminate non-modified cells (except wild type condition), then cultured with 2000 ng/ml dox for expression of the vector gene of interest or no dox as a control. Passage number and split ratio were used to determine fold expansion of total cells from the starting well and shown for 6 weeks of culture