Fig. 4.

G6PD overexpression limits autophagy induction by Lapatinib. a IF and flow cytometry for LysoTracker on MCF7^mock and MCF7^G6PD+ after polydatin (20 μM), lapatinib (20 μM) and their combination. Flow cytometry histograms show median fluorescence. G6PD+ modified cells were not responsive to both polydatin and lapatinib. b IF and flow cytometry for LC3B on MCF7^mock and MCF7^G6PD+ after polydatin, lapatinib and their combination. Flow cytometry histograms show median fluorescence. MCF7^G6PD+ cells were not responsive to both polydatin and lapatinib. Both IFs are example of three biological replicates that show similar results. c Immunoblot and band densitometry for LAMP1, p62, LC-3 on both MCF7^mock and MCF7^G6PD+ treated with Lapatinib, polydatin and combinations of both drugs. *p < 0.05, N = 3 (biological replicates); error bars = SEM