Table 2.
Primer sequences for quantitative RT PCR
| Gene name | Primer sequence (5′ → 3′) | Amplicon length [bp] | ||
|---|---|---|---|---|
| Forward | Reverse | |||
| Pde3a | NC_000072.6 Reference GRCm38.p4 C57BL/6J | TTTAACGCCAAGGTAAACGATG | TGATGTCAGCCAGCTTTATACA | 97 |
| Pde3b | NC_000073.6 Reference GRCm38.p4 C57BL/6J | GAAAAAGTGCCTGTGATCAGAC | TCTGTTCTCGGGAAATACAAGG | 130 |
| Pde4a | NC_000075.6 Reference GRCm38.p4 C57BL/6J | GCTGACCTGAAGACTATGGTAG | GATACGGTCAGAGTAGTTGTCC | 81 |
| Pde4b | NC_000070.6 Reference GRCm38.p4 C57BL/6J | CAGGAAAATGGTGATTGACATGGTGTTGG | CGAAGAACCTGTATCCGGTCAGTATAG | |
| Pde4d | NC_000079.6 Reference GRCm38.p4 C57BL/6J | CCAGAATCTGACCAAAAAGCAA | GATCCTGTCGGAGTAGTTATCC | 175 |
| Atp2a2 | NC_000071.6 Reference GRCm38.p4 C57BL/6J | TTCTGCTTATCTTGGTAGCCAA | CTTTCTGTCCTGTCGATACACT | 125 |
| Sln | NC_000075.6 Reference GRCm38.p4 C57BL/6J | ATGGAGAGGTCTACTCAGGAGCTG | ACCTCACGAGGAGCCACATAAGG | 82 |
| Adrb2 | NC_000084.6 Reference GRCm38.p4 C57BL/6J | CACAAAGCCCTCAAGACTTTAG | CCTGATAACGTGCACGATATTG | 93 |
| Ryr2 | NC_000079.6 Reference GRCm38.p4 C57BL/6J | CCATTGAGAACAAGTACATGCC | AATTCGTTGTTCATCATGAGCC | 112 |
| Plb | NC_000076.6 Reference GRCm38.p4 C57BL/6J | TACCTCACTCGCTCGGCTATCAG | CACAATGATGCAGATCAGCAGCAG | 132 |
| Cacna1c | NC_000072.6 Reference GRCm38.p4 C57BL/6J | GAGACAATGTGTGGAATATGCC | GTAGGTAGAGTTGACCACGTAC | 103 |