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. 2019 Feb 26;10(4):639–643. doi: 10.1021/acsmedchemlett.8b00608

Figure 2.

Figure 2

Antiproliferative and molecular effects of chalcones and chalcone-like derivatives in KOPTK1 T-ALL cells. (a) Protein expression levels of activated domain of Notch1 (N1Val) in KOPTK1 cells treated for 36 h with 2.5 μM of compounds 1–18 or vehicle alone (DMSO). β-Actin is used as loading control. (b) Histogram shows the mean of relative cell viability of KOPTK1 cells treated with 2.5 μM of compounds 1–18 or DMSO for 36 h measured with MTS assay ± SEM obtained from two independent experiments performed in triplicate. ****P < 0.0001, comparing with DMSO. (c) Representative graph showing the results of Pearson’s correlation test between the average values of relative N1Val expression obtained with optical densitometry and cell viability in KOPTK1 treated with compounds 1–18 vs DMSO. R = 0.9535, R2 = 0.90925, p < 0.0001. (d) N1Val and β-actin protein expression levels (lower panel) and DELTEX1 (DTX1) relative gene expression levels (upper panel) in KOPTK1 cells treated with 2.5 μM of compounds 8, 1, 5, 9, 12, and 18 or vehicle alone (DMSO) for 36 h. Data represent mean values for three independent experiments performed in triplicate ± SEM. (e) IC50 values of compounds 8, 1, 5, 9, 12, and 18 determined in KOPTK1 after 36 h of incubation. Data represent mean values for three independent experiments performed in triplicate ± SD. (f) Protein expression of p27kip and of the nonprocessed PARP (FL PARP) and its cleaved form (CL PARP). β-Actin is used as loading control.