Table 2.
Tested carrier material.
| Abbreviation | Material | Crosslinker | Surface patterning | Collagen concentration∗ |
|---|---|---|---|---|
| HAM | Denuded HAM | — | — | — |
| RHC I | RHC I | EDC/NHS | — | ~3 mg/cm2 |
| RHC I F-μCP | RHC I | EDC/NHS | Fibronectin microcontact printing | ~3 mg/cm2 |
| CLP-12 EDC | CLP-PEG | EDC/NHS | — | 12% |
| CLP-12 | CLP-PEG | DMTMM | — | 12% |
| CLP-12F-μCP | CLP-PEG | DMTMM | Fibronectin microcontact printing | 12% |
| CLP-12 3D | CLP-PEG | DMTMM | 3D topography | 12% |
| CLP-18 | CLP-PEG | DMTMM | — | 18% |
∗Concentration of RHC I is expressed as net weight at collagen casting (mg/cm2). Concentration of CLP is expressed as percentage of stock solute (%). —: not applicable; HAM: human amniotic membrane; RHC I: recombinant human collagen type I; CLP-PEG: PEGylated collagen-like peptide; EDC: 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide; NHS: N-hydroxysuccinimide; DMTMM: 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride.