Figure 4.
L. (L.) amazonensis-specific antibody production in VDD mice. C57BL/6 and BALB/c mice normally fed (Ctrl) or on a Vitamin D-deficient diet (VDD) were subcutaneously infected in the footpad with 2 × 105 L. (L.) amazonensis promastigotes. On day 92 (C57BL/6) or 99 (BALB/c) post-infection, L. (L.) amazonensis-specific antibody production in the serum was evaluated by ELISA. IgG1 (A,B), IgG2a (C,D), IgA (E,F), and IgE (G,H) levels were quantified. Mean ± SD; n = 5. The data (means ± SD; n = 5; *P < 0.05) are representative of two independent experiments producing the same result profile.