A,B) Representative micrographs from coronal sections of C57BL/6 mice treated with saline (A) or two doses DSP-4 (50 mg/kg) and immunostained with an antibody against tyrosine hydroxylase in locus coeruleus. IV, fourth ventricle. Scale bars, 100 μm. C) TH+ cell counts in animals administered vehicle or varying doses of DSP-4 (one dose of 50 mg/kg (p = 0.003) or 75 mg/kg (p < 0.001), or 2 doses of 50 mg/kg (p < 0.001); n = 3 per group). D) TH+ cell counts in animals which underwent maximal electroshock with citalopram 20 mg/kg (p < 0.001) or vehicle pre-treatment (p < 0.001, p = 0.072 between vehicle and citalopram groups) one week after the second of two 50 mg/kg DSP-4 doses (n = 7–8 per group), as indicated on Table 1. *, p < 0.05. E) Percentage of saline and DSP-4 treated mice surviving seizures induced by MES following treatment with vehicle (p = 0.02 vehicle with DSP-4 compared to citalopram without DSP-4) or citalopram (20 mg/kg, p = 0.001 citalopram with DSP-4 compared to citalopram without DSP-4). n = 8 per group. *, p < 0.05.