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Caspase-3 specificity of radiotracer candidates. 1 mM of 3 (a), 11 (b), 13 (c), 15 (d), 16 (e), or 20 (f) was incubated with a panel of caspase enzymes at 37 °C for 2 h. Cleavage products were measured by HPLC and peak areas were compared with a standard curve of 2MP-VD-OH or 2MP-TbD-OH to calculate the yield of hydrolyzed product in ng. Bars represent mean ± standard deviation, n = 3.
