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. Author manuscript; available in PMC: 2020 Jan 15.
Published in final edited form as: Bioorg Med Chem Lett. 2018 Nov 28;29(2):204–211. doi: 10.1016/j.bmcl.2018.11.055

Figure 6. C17 reduces the levels of Mdm2 and cause the accumulation of P53 and P21 in MCF7 cells.

Figure 6.

Intervening lanes were removed for clarity. A. MCF7 cells were treated with C17 (50 μM) for 4 h and K48 ubiquitin chains were measured by immunoblotting. B-F. MCF7 cells were treated with C17 for 4 h and the levels of Mdm2 (B), p53 (C, D and E) and p21 (F) were measured by immunoblotting. Note: error bars in E are too small to be clearly observable. G. MCF7 and B16/F10 cells were treated with C17 every 24 h. After 72 h, viable cells were measured by Alamar Blue®. H. The effects of C17 on the cell cycle as measured by FACS. I. The viability of MCF7 cells treated with products of C17 hydrolysis.