. Author manuscript; available in PMC: 2020 Jan 15.

Published in final edited form as: Bioorg Med Chem Lett. 2018 Nov 28;29(2):204–211. doi: 10.1016/j.bmcl.2018.11.055

Table 2.

Kinetic parameters for the inhibition of recombinant USP7 catalytic domain (USP7CD) and USP9x.

Cmpd	USP7CD	USP9x
Cmpd	k_on (M⁻¹ s⁻¹)	k_on (M⁻¹ s⁻¹)
C14	13 ± 4	18 ± 2
C15	8 ± 3	6 ± 1
C17	9 ± 4	49 ± 3
C18	NI	17 ± 1
C20	21 ± 2	NI
C22	NI	58 ± 3
WP1130	66 ± 4	50 ± 30
b-AP15	n.d.	140 ± 10
PR619	n.d.	90 ± 20
D12 prost	n.d.	NI
BITC ^a	n.d.	170 ± 40
PEITC ^a	n.d.	150 ± 20

Data were fit to a single step inactivation mechanism as described in Material and Methods. NI, no inhibition; n.d., not determined. The values of k_on for benzylisothiocyanate (BEITC) and phenethylisothiocyante (PEITC) are included for comparison.

^a.

Values from ¹³.