. Author manuscript; available in PMC: 2019 Jun 5.

Published in final edited form as: Biochemistry. 2018 Mar 26;57(22):3146–3154. doi: 10.1021/acs.biochem.8b00142

Table 1. Effect of D₂O on ³¹P dipolar relaxation parameters for substrate and cofactor in GMPR ternary complexes extracted from field cycling relaxometry.

Values for τ, R₀ and τ/R₀ are the average and standard deviation from 3 independent experiments.

³¹P	Complex	Solvent	τ (ns)	R₀ (s⁻¹)	τ/R₀ × 10⁸ (s²)
IMP	WT•IMP •NADP⁺	H₂O ^a	55 ± 12	1.9 ± 0.9	3.3 ± 1.1
IMP	WT•IMP •NADP⁺	D₂O	45 ± 1	0.6 ± 0.2	8 ± 3
GMP	WT•GMP •NADP⁺	H₂O ^a	72 ± 13	0.30 ± 0.02	24 ± 5
GMP	WT•GMP •NADP⁺	D₂O	77 ± 16	0.3 ± 0.1	24 ± 12
NADP⁺ mono-phosphate	WT•IMP •NADP⁺	H₂O ^a	103 ± 23	2.9 ± 1.2	3.8 ± 0.9
	WT•IMP •NADP⁺	D₂O	62 ± 3	0.7 ± 0.3	9 ± 4
	WT•GMP •NADP⁺	H₂O ^a	47 ± 11	0.6 ± 0.1	8.6 ± 2.3
	WT•GMP •NADP⁺	D₂O	55 ± 18	0.4 ± 0.1	13 ± 5
NADP⁺ diphosphates	WT•IMP •NADP⁺	H₂O ^a	88 ± 13	1.4 ± 0.3	6.6 ± 1.2
		H₂O ^a	82 ± 17	1.6 ± 0.3	5.1 ± 0.9
		D₂O	70 ± 5	0.6 ± 0.3	12 ± 7
		D₂O	58 ± 8	0.6 ± 0.3	10 ± 5
	WT•GMP •NADP⁺	H₂O ^a	44 ± 5	0.5 ± 0.2	9.2 ± 2.4
		H₂O ^a	45 ± 8	0.5 ± 0.1	9.3 ± 2.5
		D₂O	50 ± 9	0.3 ± 0.1	15 ± 5
		D₂O	50 ± 1	0.3 ± 0.1	15 ± 3

Values from ⁴.

Table 1. Effect of D2O on 31P dipolar relaxation parameters for substrate and cofactor in GMPR ternary complexes extracted from field cycling relaxometry.