Skip to main content
. 2019 Apr 16;14(4):e0215062. doi: 10.1371/journal.pone.0215062

Fig 4. The intrabody inhibits recombinant IFNα4 secretion by co-transfected HEK293T cells.

Fig 4

Serial dilutions of supernatants of HEK293T cells transiently co-transfected with Twin-Strep-tagged IFNα4 expression plasmid and αIFNα-ib or control intrabody expression plasmids or empty vector pCMV/myc/ER were applied to ELISA plates immobilized with purified anti-IFNα hybridoma antibody 4EA1. Secreted IFNα4 was detected with anti-StrepTag peroxidase-labelled antibody. Bars indicate standard deviations (SD) calculated from two independent experiments. IFNα4 concentration differences between the 1:32 diluted supernatants of the αIFNα-ib plasmid-treated cells (asterisk) and supernatants treated with any of the three other plasmids were statistically significant with p < 0.05 (n = 2, one way ANOVA followed by Holm-Sidak test).