Fig 6. Subcellular colocalization of intrabody and endogenous IFNα in stably transfected macrophages and dendritic cells after stimulation with Poly (I:C).

RAW-αIFNα-ib-eGFP macrophages and D1-αIFNα-ib-eGFP dendritic cells were stimulated for 24 hours with Poly (I:C). After fixation and permeabilization of cells, endogenous IFNα, αIFNα-ib and calnexin were stained with rat monoclonal anti-IFNα F18, mouse anti-myc and rabbit anti-calnexin, followed by incubation with goat anti-rat Cy3, goat anti-mouse AMCA and goat anti-rabbit Cy5. Merged images of IFNα and intrabody, and of all three proteins were generated.