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D1-αIFNα-ib-eGFP cells, control D1-eGFP cells and D1-wt cells were stimulated for 24 h with Poly (I:C), then fixed and permeabilized. Expression of eGFP was detected by its green fluorescence. Endogenous IFNα was stained with rat anti-IFNα F18 and goat anti-rat Fc Alexa Flour594-labelled antibodies and αIFNα-ib with mouse anti-c-myc and goat anti-mouse IgG (Fc specific) Cy3-labelled antibodies.
