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. 2019 Apr 16;14(4):e0215062. doi: 10.1371/journal.pone.0215062

Table 2. Cloning PCR primers.

Primer Sequence
CMV-for CGC AAA TGG GCG GTA GGC GTG
scFv-AKG-SP gcc aga ctc cac cag ctg cac ttc GGA Gtg cac gcc tgt gga gag aaa ggc aaa g
scFV-AKG-VH-S-G gaa gtg cag ctg gtg gag tct ggc gga ggc tta gta cgg c
scFv-AKG-VH-AS tga gga gac tgt gac cac gac tcc
scFV-AKG-Linker gga gtc gtg gtc aca gtc tcc tca GGT GGA GGC GGT TCA GGC GGA GGT GGC TCT GGC GGT GGC GGA TCG gac act gtg ctg acc cag tct cct
scFV-AKG-VL-AS gtG CGG CCG Ctt tca att cca cct tgg tgc ctg a
IB-LV-for tcgacggatcgggagatc
IB-LV-rev actatGTCGACtacagctcgtccttctc
SEW-up CAC AAC CCC TCA CTC GGC GCG CCA GTC CTC CGA CAG ACT GAG TCG CCC GGG GGG GAT CCA CCG GTC GCC ACC ATG GGT GAC AAT GAC ATC CAC T
SEW-down TAT CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGT CGC GGC CGC TTT ACT
TGT ACA GCT CGT CCA
IFNA4-S-Esp3I CGT CTC Tct ccT GTG ACC TGC CTC ACA CTT ATA AC
IFNA4-AS-Tag CGT CTC Ttc gaG CTC CTT CTC CTC ACT CAG TCT TG