Table 2. Cloning PCR primers.
Primer | Sequence |
---|---|
CMV-for | CGC AAA TGG GCG GTA GGC GTG |
scFv-AKG-SP | gcc aga ctc cac cag ctg cac ttc GGA Gtg cac gcc tgt gga gag aaa ggc aaa g |
scFV-AKG-VH-S-G | gaa gtg cag ctg gtg gag tct ggc gga ggc tta gta cgg c |
scFv-AKG-VH-AS | tga gga gac tgt gac cac gac tcc |
scFV-AKG-Linker | gga gtc gtg gtc aca gtc tcc tca GGT GGA GGC GGT TCA GGC GGA GGT GGC TCT GGC GGT GGC GGA TCG gac act gtg ctg acc cag tct cct |
scFV-AKG-VL-AS | gtG CGG CCG Ctt tca att cca cct tgg tgc ctg a |
IB-LV-for | tcgacggatcgggagatc |
IB-LV-rev | actatGTCGACtacagctcgtccttctc |
SEW-up | CAC AAC CCC TCA CTC GGC GCG CCA GTC CTC CGA CAG ACT GAG TCG CCC GGG GGG GAT CCA CCG GTC GCC ACC ATG GGT GAC AAT GAC ATC CAC T |
SEW-down |
TAT CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGT CGC GGC CGC TTT ACT TGT ACA GCT CGT CCA |
IFNA4-S-Esp3I | CGT CTC Tct ccT GTG ACC TGC CTC ACA CTT ATA AC |
IFNA4-AS-Tag | CGT CTC Ttc gaG CTC CTT CTC CTC ACT CAG TCT TG |