Figure 3.
Protein expression of the WT and the six GR variants. (A) HEK293T cells were transiently transfected or not with a plasmid encoding the WT or the six GR variants. Twenty-four hours posttransfection, protein extracts (20 µg) were analyzed by western blot followed by immunostaining with an antibody recognizing the NTD of GR (29; dilution 1/500) and an anti‒β-actin antibody (30; dilution 1/1000). GRα protein expression was similar between the WT and the six variants but was below the detectable threshold in the untransfected cells used as control (i.e., C). Representative image of three independent experiments is shown. The molecular weight marker used was PageRulerTM (Thermo Fisher Scientific). (B) Quantification of the protein signal did not reveal any statistically significant difference between the WT and the GR variants. Results are expressed as means ± SEM.