Table 2.

Primer Sequences Used for Site-Directed Mutagenesis to Generate the Six Genetic GR Variants

Variants	Primer Sequence
F65V	5′-CGACGCAAGACTTTTGGTTGATGTTCCAAAAGGCTCA-3′
M86V	5′-TCTGTCAAAGCAGTTCCACTCTCAGTGGGACTGTATAT-3′
A229T	5′-TTTGCTTTCTCCTCTGACGGGAGAAGACGATTCA-3′
A304E	5′-CACAGTTTACTGTCAGGAAAGCTTTCCTGGAGCAA-3′
N374S	5′-GGATCTGGAGATGACAGCTTGACTCTCTGGGG-3′
R386Q	5′-CTCTGAACTTCCCTGGTCAAACAGTTTTTTCTAATGGC-3′
N374E	5′-GAGTCCCCAGAGAAGTCAATTCGTCATCTCCAGATCCTTGG-3′

The base in bold indicates the point mutation.