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. 2019 Apr 16;14(4):e0215472. doi: 10.1371/journal.pone.0215472

Fig 6. Correlation analysis of relative expression levels determined by RNA-seq and qPCR on selected genes NADP-ME (MDP0000568928), NAD-MDH (MDP0000174740), PEPCK (MDP0000661864) and PEPC (MDP0000073810).

Fig 6

All qPCR reactions were carried out by using three independent biological replication and repeated three times. Relative expression levels were calculated from CT values according to the 2-ΔΔCT method. Actin was used as the reference gene for these qPCR experiments.