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. 2019 Apr 5;146(7):dev175570. doi: 10.1242/dev.175570

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© 2019. Published by The Company of Biologists Ltd

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Fig. 2. — The U1-U5 motor neurons extend axons and dendrites sequentially. (A-H) Axon outgrowth timing in early- and late-born neurons of the NB7-1 lineage in stage 13-15 embryos. (A-B) Wild-type multicellular MCFO labeling (NB7-1-Gal4^KZ UAS-MCFO). Analysis was restricted to lineages in which early-born medially located neurons were stochastically labeled in one MCFO color, and in which the later-born laterally located neurons were stochastically labeled in a different MCFO color. (A) All labeled cells. (A′-A‴) Early-born medial neurons (green) project out of the CNS ahead of later-born lateral neurons (magenta). (B) Quantification of axon length as a representation of timing of axon outgrowth; early-born neurons project further (earlier) than late-born neurons (***P<0.001). (C-D) Hb misexpression (NB7-1-Gal4^KZ UAS-hb UAS-MCFO) multicellular MCFO labeling. (C) All labeled cells. (C′-C‴) Early-born medial neurons (green) project out of the CNS ahead of later-born lateral neurons (magenta). (D) Quantification of axon length as a representation of timing of axon outgrowth; early-born neurons project further (earlier) than late-born neurons (***P<0.001). (E-F) Wild-type single-neuron MCFO labeling (NB7-1-Gal4^KZ UAS-MCFO). (E) A single early-born medial neuron (green) always projects out of the CNS ahead of a single later-born lateral neuron (magenta). (F) Quantification of axon length as a representation of timing of axon outgrowth; early-born neurons project further (earlier) than late-born neurons (***P<0.001). (G-H) Hb misexpression (NB7-1-Gal4^KZ UAS-hb UAS-MCFO) single-neuron MCFO. (G-G″) A single early-born medial neuron (green) always projects out of the CNS ahead of a single later-born lateral neuron (magenta). Quantification of axon length as a representation of timing of axon outgrowth; early-born neurons project further (earlier) than late-born neurons (***P<0.001). (I-L) Dendrite outgrowth timing in early- and late-born neurons of the NB7-1 lineage in stage 13-15 embryos. (I-I″) Wild-type single-neuron MCFO labeling (NB7-1-Gal4^KZ UAS-MCFO). A single early-born medial neuron (green) extends a dendrite before a single later-born lateral neuron (magenta). (J) Quantification of axon length as a representation of the timing of axon outgrowth; early-born neurons project further (earlier) than late-born neurons (***P<0.001). (K-K″) Hb misexpression (NB7-1-Gal4^KZ UAS-hb UAS-MCFO) single neuron MCFO labeling. A single early-born medial neuron (green) extends a dendrite before a single later-born lateral neuron (magenta). (L) Quantification of axon length as a representation of timing of axon outgrowth; early-born neurons project further (earlier) than late-born neurons (P<0.001). Arrowheads indicate the early-born axon (green) and late-born axon (magenta); dashed line indicates the midline. Scale bars: 5 μm.