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. 2019 Apr 5;146(7):dev175570. doi: 10.1242/dev.175570

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© 2019. Published by The Company of Biologists Ltd

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Fig. 5. — Ectopic U1 axons project to dorsal muscles and lack ventral muscle targets. (A-B‴) Wild-type (NB7-1-Gal4^KZ UAS-GFP) and Hb-misexpression (NB7-1-Gal4^KZ UAS-hb UAS-GFP) L1 larvae stained for U motor neurons (green) and muscles (magenta). (A-A‴) Wild type: U motor neurons project axons to dorsal muscles (DO1/DO3) and more ventral muscles in the LL1/DA3 region. (B-B‴) Hb misexpression: U motor neurons project only to dorsal muscle targets (magenta, shown in B′) consistent with ectopic U1-U2 identity at the expense of U3-U5 neuronal identity. Arrowheads indicate U1-U2 muscle targets (green) and U3-U5 muscle targets (red). (A″″) Quantification. (C-C″,D-D″,E-E″) Hb misexpression L1 larvae (NB7-1-Gal4^KZ UAS-hb UAS-MCFO) showing MCFO-labeled single neurons. (C-C″) The endogenous U1 motor neuron (green; closest to midline). (C) Dorsal view showing medial cell body position, contralateral dendrites and ipsilateral axon (Zfh2 negative; not shown). (C′) Cross-sectional view of the same U1 neuron. (C″) Dorsal view of the body wall showing the U1 axon (green arrowhead) projecting to the most dorsal extent of the FasII⁺ motor neurons (magenta arrowhead). (C‴) Quantification. (D-D″) An ectopic U1 motor neuron (green). (D) Dorsal view showing lateral cell body position, contralateral dendrites and ipsilateral axon (Zfh2 negative; not shown). (D′) Cross-sectional view of the same neuron; the contralateral dendrite has a dorsal origin and there is a lack of bipolar morphology. (D″) Dorsal view of body wall showing the ectopic U1 axon (green arrowhead) projecting to the most dorsal extent of the FasII⁺ motor neurons (magenta arrowhead). (D‴) Quantification. (E-E″) A late-born laterally positioned U3, U4 or U5 motor neuron that was not transformed (based on being Zfh2⁺; not shown). (E) Dorsal view showing far lateral cell body position, ipsilateral dendrites and ipsilateral axon. (E′) Cross-sectional view of the same neuron. (E″) Dorsal view of the body wall showing the U3-U5 axon (green arrowhead) projecting to a more ventral region along the FasII⁺ motor neurons (magenta arrowhead). (E‴) Quantification.