Fig. 3.
miR-203 sponge vector causes premature NCC delamination. (A) Schematic diagram of the pSmiR-203 sponge vector with bulged miR-203 antisense sequences downstream of the EGFP reporter. (B) Embryos electroporated with pSmiR-203 and miR-203 MO have premature migration of NCCs, as shown by in situ hybridization for Sox10 and immunostaining for SNAIL2 and FOXD3, compared with the uninjected side of the same embryos or injected (right) with pSmiR-scramble vector or control MO. Arrowheads indicate premature migratory NCCs. (C) Quantitation of premature NCC migration in pSmiR-203-, pSmiR-scramble-, control MO- and miR-203 MO-treated embryos. Numbers in the graph represent the analyzed embryos. ****P<00001 (contingency table followed by χ2 test).