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. 2019 Apr 11;146(7):dev171017. doi: 10.1242/dev.171017

Fig. 4.

Fig. 4.

Snail2 and Phf12 3′UTRs are direct targets of miR-203. (A) Schematic diagram of a dual-colored sensor vector containing wild or mutated (mt) 3′UTRs from Snail2 (pUTR-Snail2) and Phf12 (pUTR-Phf12). pCAG, chick β-actin promoter; d4EGFPN, nuclear-localized destabilized EGFP with a half-life of 4 h; mRFPN, nuclear-localized monomeric red fluorescent protein. (B) Diagram of electroporation assays for 3′UTR sensor experiments. Electroporation of sensor vector containing the 3′UTRs for (C) Snail2 (pUTR-Snail2) or (D) Phf12 (pUTR-Phf12) together with miR-203-overexpressing vector (pCAG-203) causes a consistent reduction on the d4EGFPN expression (right side) compared with the control side (left). (C,D) Mutation of miR-203-binding sites in the 3′UTRs of Snail2 (pUTR-mtSnail2) or Phf12 (pUTR-mtPhf12); most of the electroporated cells are yellow, expressing both d4EGFPN and mRFPN, even when miR-203 is overexpressed (right side).