Fig. 2.

DSS-induced muscle damage is similar to muscle damage from loss of basement membrane. (A) Schematic representation of the linkage between the actomyosin contractile machinery and the basement membrane. The linkage between the basement membrane and the muscles can occur through integrins or dystroglycans. (B,C,E,F) Phalloidin staining revealed sarcomeres in longitudinal gut muscles. Yellow brackets indicate sarcomeres. Scale bar: 5 µm. (D) Knockdown of LanB1 (LanB1-KD) ubiquitously using TubP-Gal4 reduced sarcomere size ∼10% compared to control. Sarcomeres were measured in five flies for each condition. (G) Feeding flies DSS reduced sarcomere size ∼30% compared to control. Sarcomeres were measured in four control and six DSS-fed flies. See also Fig. S2 for sarcomere length in relaxing buffer. Data in D,G show mean±s.e.m. P-values calculated by unpaired t-test.