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. 2019 Apr 8;132(7):jcs226860. doi: 10.1242/jcs.226860

Fig. 6.

Fig. 6.

Basement membrane is repaired 48 h after termination of DSS feeding. (A) Muscle morphology was analyzed at the indicated times after withdrawing animals from DSS food to normal food. The muscle aspect ratio was restored at 48 h after terminating DSS feeding. Four to eight flies were analyzed for each time point. (B–D) TEM image of muscles and basement membrane of the midguts in a control-fed fly (A), DSS-fed fly (B), and a fly that recovered on normal food for 48 h after DSS feeding (C). Basement membranes are indicated with yellow arrows. Both muscle morphology and basement membrane thickness have been repaired by 48 h after termination of DSS feeding. (E) Quantification showing repair of basement membrane thickness 48 h after termination of DSS feeding, measured on TEM micrographs. Scale bar: 1 µm. Data in A,E show mean±s.e.m. P-values calculated by ANOVA to determine significance followed by unpaired t-tests with Bonferroni correction. NS, not significant.