Fig. 8.

Basement membrane repair requires new Collagen IV and Laminin. (A–I) Epifluorescence microscopy images of Vkg–GFP (A–C,G–I) or LanB1–GFP (D–F) showing the basement membrane and muscle morphology before, during or after DSS feeding. When vkg (vkg-KD) or LanB1 (LanB1-KD) is knocked down in adults using TubP-Gal4, Gal80ts, basement membranes do not repair as inferred from the muscle morphology (F,I). Scale bar: 10 µm. (J) Quantification of the muscle aspect ratio. Four to six flies were analyzed for all conditions, except three flies for the LanB1-KD 48 h sucrose control. Data shows mean±s.e.m. For statistical analysis of significance, see Table S3. The repair-deficient phenotype was observed with second RNAi lines targeting vkg or LanB1 (not shown).