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. 2019 Apr 3;132(7):jcs223925. doi: 10.1242/jcs.223925

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — External nucleotides regulate human mTORC1 and growth rates. (A) Western blot analysis and quantification of AMPK and TORC1 substrate (S6K) phosphorylation in extracts from control HEK293T cells (+Gln) or HEK293T cells after transfer into the glutamate starvation medium for 60 min (Gln starved) and treatment with 2 mM ATP. Samples were taken at the indicated time point after ATP addition (′ indicates minutes). Relative changes compared to non-treated cells with standard deviation are shown in the graph, n=3. (B) Growth assay showing cell sensitivity to ATP and AMP. Average growth relative to non-treated along with standard deviation of n=3 experiments is shown. *P≤0.05 (two-tailed Student's t-test).