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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Mol Nutr Food Res. 2018 Apr 26;62(10):e1700932. doi: 10.1002/mnfr.201700932

Figure. 1. Butyrate suppresses proliferation and induces apoptosis in HT-29 and Caco-2 cells.

Figure. 1.

Cells were treated with 0 (□) or 4 mM sodium butyrate (NaB, ■) for 4 d. (A) Immunofluorescence staining of PCNA for cell proliferation. Scale bars are 200 μm. (B) Caco-2 cells were transfected with GFP plasmid, and observed under a fluorescent inverted microscope. Scale bars are 200 μm on left and 100 μm on right. (C) MTT analyses for cell survival. (D) Protein contents of cleaved caspase-3 and cleaved PARP. Mean ± SEM, n = 3, *: P < 0.05; **: P < 0.01.