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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: J Mol Histol. 2018 Dec 8;50(1):63–73. doi: 10.1007/s10735-018-9807-y

Figure 4. DHCR7, DHCR24 and EBP proteins show comparable expression in the nuclear envelope.

Figure 4.

Neuro2a cells were transiently transfected with EGFP-DHCR7, EGFP-DHCR24 and ptdTomato-EBP fusion constructs. Cells were labeled with anti-emerin (localizing to the ER and NE inner and outer membranes) antibody and analyzed by confocal microscopy. Nuclei were visualized by Hoechst dye. Original images were collected with a 60× oil objective. Calibration bar, 5 μm. Note that all three recombinant proteins co-localized with emerin in the nuclear envelope, and ER localization for all three proteins was similar to that observed previously with anti-PDI labeling.