Fig. 13.
Localization of midbrain Pnoc and Vgat neurons projecting to the VTA in transgenic Pnoc::ZsGreen and Vgat::ZsGreen mice. a-e. Neurons retrogradely labeled (CTB) from the VTA are observed throughout the midbrain. Neurons double-labeled for CTB and Pnoc::ZsGreen are concentrated in the RMTg (magenta dashed outlines). f-k. Neurons double-labeled for CTB and Vgat::ZsGreen are also concentrated in the RMTg and overlap with LHb efferents. The yellow shaded region indicates efferents anterogradely labeled from LHb via AAV injection into LHb (injection area indicated in f). Translucent blue shows location where heavy CTB fiber expression precluded accurate neuronal labeling.