Fig. 8.

Pnoc neurons express Gad1 in rats. a-g. Pnoc mRNA labeling is heavy (greater density of ISH signal, i.e., silver grains) in neurons within the RMTg region (magenta dashed outlines). h-n. Gad1 mRNA (via DIG-labeled ISH) is observed in the RMTg but also to a similar degree in surrounding areas. o-u. Neurons double-labeled for heavy Pnoc and Gad1 are more common within the RMTg as compared to outside the RMTg. Notably, both within and outside the RMTg, almost all Pnoc neurons express Gad1. However, Gad1 neurons expressing Pnoc are only common within the RMTg. Scalebar = 1 mm.