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. 2019 Apr 16;8:e45019. doi: 10.7554/eLife.45019

Figure 3. At folding initiation the EGL has uniform proliferation, cell size, and cell shape.

(A,B) Low and high power images of immuno-histochemical (IHC) staining of sagittal cerebellar sections to measure proliferation in the lobules (L) indicated at 25 μm windows surrounding the ACs (stars). Scale bar: 200 μm. (C,D) EGL proliferation rates are shown before and during the onset of invagination (Two-way anova: df = 2. (C) p=0.10, F = 4.36 (D) p=0.03, F = 10.31). (E,F) Proliferation rates are shown in the AC and in the surrounding EGL showing uniformity (Two-way anova: df = 18. (E) p=0.03, F = 2.15 (F) p=2.1e−3, F = 3.06). (G) Section of Atoh1-CreER/+; R26MTMG/+ E16.5 cerebellum showing masked labeled cells. Scale bar: 20 μm. (H,I) Cell shape (sphericity) measurements before and during folding (anova df = 3. (H) p=0.34, F = 1.13 i p=0.61, F = 0.61). (J,K) Cell size measurements before and during folding (anova df = 3. (J) p=3.6e−3, F = 4.75, (K) p=0.85, F = 0.26). Stars indicate statistical differences. Error bars: S.D.

Figure 3.

Figure 3—figure supplement 1. Proliferation rate is reduced in the central zone of the cerebellum after folding initiation.

Figure 3—figure supplement 1.

(A) Proliferation is reduced in lobule region L678 compared to other lobules (anova: df = 2; p=0.01, F = 9.24). (B) Proliferation through the AC regions is uniform with the surrounding EGL (anova: df = 10; p=0.17, F = 1.64). (C) At E18.5 cells within the EGL are smaller than at earlier stages (two-tailed t-test df = 330 P=2.92e−20, T = 9.85). Stars: statistical differences. Error bars: S.D.