Figure 2.

Pol δ shows strand displacement activity on the 72:17-mer minicircular template-primer. (A) Pol δ (0.1 unit), was incubated in the presence of 12 nM (3′-OH ends) 72:17-mer template-primer under the conditions described in Materials and Methods. Reactions were stopped at the indicated times and the products resolved on a 14% PAA/7 M Urea gel. Lane M: 5′-labeled linear 70-mer and 17-mer were loaded as markers. (B) Sizes of the synthesized products in nt were calculated from their relative electrophoretic mobility and plotted versus time. Data points were fitted to a mixed exponential equation by computer simulation. (C) Length (in nt) of the displaced strand was calculated from the size of the synthesized products and plotted versus time. The solid line represents the best linear interpolation of the data points.